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K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium
glucose and agar
2 at 25°C Suspend 23 gram powdered medium in 1 litre distilled water
YM Broth and YM Agar can be acidified to pH 3
AX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells
HL5 Medium without Glucose supplemented with vitamins and micro elements Size:1000g K12 has been ultimately modifiedFormula g l Peptone 14 Yeast Extract 7 KH2PO4 0. 5 Na2HPO4 0. 5 FM Vitamins and Micro elements 0. 01 Suspend 22 gram powdered medium in 1 litre distilled water Store dry at room temperature
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