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EMM containing 0
K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium
To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium
1 g/L SDS 69
Adams and R
Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) EMM containing 0Containing all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 32. 9 grams l with Neidhardt supplement mixture in a concentration of 3. 1 grams l. Suspend 32. 9 grams of Neidhardt basal salt mixture plus 3. 1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this
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