Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:500g 3g in 1L of Distilled

3g in 1L of Distilled or De-ionised Water

0 gram powdered medium in 1 litre distilled water

Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation

Dilute 100ml TBE Buffer 10X into 900ml deionised water to make 1 litre of TBE Buffer

This makes a final concentration of 50mM MOPS

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:500g 3g in 1L of DistilledBoric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room